Abstract: Objective　To initially establish a scoring standard on compliance for beagle dogs and explore the correlation between beagle dogs’ compliance and the c.264G>A SNP site of tyrosine hydroxylase (TH) gene and plasma 5-hydroxytryptamine (5-TH).Methods　By evaluating indicators such as the closeness between beagle dogs and humans, experimental cooperation, and convenience of feeding management, a preliminary scoring standard for beagle dogs’ compliance was established. Based on compliance assessment from 350 beagle dogs high and low compliance dogs (10 each) were selected.  The C264 SNP site of TH gene was analyzed by polymerase chain reaction sequencing method and plasma 5-HT concentration was determined by enzyme-linked immunosorbent assay kit. The correlation analyses were performed to explore the compliance with c.264G>A SNP site of TH gene and 5-HT concentration. Results　The dogs in the high compliance group were mostly GG genotypes according to the c.264G>A SNP site of TH gene and the 5-HT concentrations were higher [(32.73±8.64) mg/mL], while the dogs in the low compliance group were mostly AA genotypes and the 5-HT concentration were lower [(20.31± 6.99) mg/mL]. There was a significant correlation between compliance of beagle dogs and 5-HT concentration (P<0.05).Conclusion　The compliance of beagles shows a certain relationship with the c.264G>A SNP variation in the TH gene, although the difference is not statistically significant. However, it exhibits a significant correlation with plasma 5-HT concentration. Both the TH gene c.264G> A SNP and plasma 5-HT concentration may potentially serve as indicators and molecular markers for assessing tractability in beagles.

Key words: beagle dog, compliance, SNP, 5-HT, TH

摘要： 目的　初步建立比格犬的顺应性评分标准,探讨比格犬顺应性与酪氨酸羟化酶(TH)基因c.264G>A SNP位 点及血浆5-羟色胺(5-HT)之间的相关性。方法　对比格犬与人亲近度、实验配合度和饲喂管理便利性等指标进行 评估,初步建立比格犬顺应性评分标准。从350只比格犬中筛选出高、低顺应性犬各10只,采用聚合酶链式反应直 接测序法分析TH基因c.264G>A SNP位点,应用酶联免疫试剂盒测定血浆5-HT浓度,对比格犬顺应性与TH基因 c.264G>A SNP位点及5-HT浓度进行关联分析。结果　高顺应性组TH基因c.264G>A SNP位点基因型多为GG 型,5-HT浓度较高[(32.73±8.64)mg/mL];低顺应性组TH基因c. 264G>A位点多为AA型,5-HT浓度较低 [(20.31±6.99)mg/mL],比格犬顺应性与5-HT浓度存在显著相关性(P <0.05)。结论　比格犬顺应性高低与TH 基因c.264G>A SNP位点变异性具有一定的关系,但差异并不显著;比格犬顺应性高低与血浆5-HT浓度具有相关 性。TH基因c.264G>A SNP位点变异性与血浆5-HT浓度或许可以作为比格犬顺应性评估指标及分子标记。

关键词: 比格犬, 顺应性, SNP, 5-羟色胺, 酪氨酸羟化酶